Skip Navigation


Journal of Heredity Advance Access originally published online on November 2, 2005
Journal of Heredity 2005 96(7):829-835; doi:10.1093/jhered/esi126
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
96/7/829    most recent
esi126v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (1)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Short, A. D.
Right arrow Articles by Ollier, W. E. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Short, A. D.
Right arrow Articles by Ollier, W. E. R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The American Genetic Association. 2005. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org.

Canine DNA Subjected to Whole Genome Amplification is Suitable for a Wide Range of Molecular Applications

A. D. Short, L. J. Kennedy, O. Forman, A. Barnes, N. Fretwell, R. Wiggall, W. Thomson, and W. E. R. Ollier

From the Centre for Integrated Genomic Medical Research, Stopford Building, University of Manchester, Manchester, UK (Short, Kennedy, Thomson, and Ollier); Mammalian Immunogenetics Research Group, Veterinary Clinical Sciences, University of Liverpool, Liverpool, UK (Barnes); Waltham Centre for Pet Nutrition, Freeby Lane, Leicestershire, UK (Fretwell and Wiggall); and Animal Health Trust, Newmarket, Suffolk, UK (Forman)

Address correspondence to Andrea D. Short at the address above, or e-mail: andrea.short{at}manchester.ac.uk.

Molecular and genetic studies of canine disease phenotypes can be limited by the amount of DNA available for analysis. New methods have been developed to amplify the genomic DNA of a species producing large quantities of DNA from small starting amounts. Whole genome amplification (WGA) of DNA is now being used in human studies, although this technique has not been applied extensively in veterinary research. We evaluated WGA of canine DNA for suitability in a range of molecular tests. DNA from 93 canine blood extracted and 18 buccal swab samples was subjected to WGA using the GenomiPhi kit (Amersham). Genomic DNA was compared with WGA product using a range of techniques, including reference strand-mediated conformation analysis, denaturing high-performance liquid chromatography analysis, microsatellite genotyping, direct DNA sequencing, and single nucleotide polymorphism allelic discrimination. All samples amplified well, giving an average yield of 3 µg of DNA from 2.5 ng of starting material. Extremely high levels of experimental reproducibility and concordance were observed between source and WGA DNA samples for all analyses used: greater than 95% for blood extracted DNA and greater than 80% for buccal swab DNA. These studies clearly demonstrate the usefulness of WGA of canine DNA as a means of increasing DNA quantities for canine studies. This technique will have major implications for future veterinary research.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Int J EpidemiolHome page
M. Amoli, D Carthy, H Platt, and W. Ollier
EBV Immortalization of human B lymphocytes separated from small volumes of cryo-preserved whole blood
Int. J. Epidemiol., April 1, 2008; 37(suppl_1): i41 - i45.
[Abstract] [Full Text] [PDF]

Home page
 J HeredHome page
M. L. Chang, R. L. Terrill, M. M. Bautista, E. J. Carlson, D. J. Dyer, K. L. Overall, and S. P. Hamilton
Large-Scale SNP Genotyping with Canine Buccal Swab DNA
J. Hered., August 9, 2007; (2007) esm044v2.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.