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Journal of Heredity Advance Access originally published online on June 15, 2005
Journal of Heredity 2005 96(7):843-846; doi:10.1093/jhered/esi090
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© The American Genetic Association. 2005. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org.

Fifty-Four New Gene-Based Canine Microsatellite Markers

M. Litt, M. L. Bestwick, M. J. Winther, and P. M. Jakobs

From the Department of Medicine, Division of Cardiology (Bestwick, Winther, and Jakobs) and Molecular and Medical Genetics (Litt) at Oregon Heath and Science University, L103A, 3181 SW Sam Jackson Park Road, Portland, OR 97239

Address correspondence to Petra Jakobs at the address above, or e-mail: jakobsp{at}ohsu.edu.

Fifty-four new markers were developed to fill in gaps in the current map of canine microsatellites and to complement existing markers that may not be sufficiently informative in highly inbred canine pedigrees. Canine genes contained on the radiation hybrid map were used to obtain the sequence of the human homolog. A BLAST search versus the canine whole genome shotgun (wgs) sequence resource was used to obtain the sequence of the canine genomic contigs containing the homolog of the corresponding human gene. Canine sequences that contained microsatellites and mapped back to the correct location in the human genome were used to design primers for amplification of the microsatellites from canine genomic DNA. Heterozygosities of the markers were tested by genotyping grandparental DNAs obtained from the Nestle Purina Reference family DNA distribution center plus DNAs from unrelated Bouviers and Irish wolfhounds. Canine map positions of markers on the July 2004 freeze of the canine genome assembly were determined by in silico PCR or BLAST.


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