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Journal of Heredity Advance Access originally published online on January 24, 2008
Journal of Heredity 2008 99(2):187-192; doi:10.1093/jhered/esm107
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© The American Genetic Association. 2008. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org.

Brief Communications

Sex Identification of Owls (Family Strigidae) Using Oligonucleotide Microarrays

Lih Chiann Wang, Lucia Liu Severinghaus, Chi Tsong Chen, Lu Yuan Liu, Chu Hsiang Pan, Dean Huang, Hsiao Yuan Lee, Jihn Tsair Lir, Shih Chien Chin, Chang En Pu, and Ching Ho Wang

From the Graduate Institute of Veterinary Medicine, National Taiwan University, 1 Sec. 4, Roosevelt Road, Taipei 106, Taiwan (L. C. Wang and C. H. Wang); Taipei Zoo, Taipei, Taiwan (L. C. Wang, Chin, Huang, and Lee); the Research Center for Biodiversity, Academia Sinica, Taipei, Taiwan (Severinghaus); the Scientific and Technical Research Center, Ministry Justice Investigation Bureau, Taipei, Taiwan (Chen and Pu); the Graduate Institute of Plant Science, National Pintung University of Science and Technology, Taipei, Taiwan (Liu); the Department of Hog Cholera, Animal Health Research Institute, Council of Agriculture, Taipei, Taiwan (Pan); and the Chung-Shan Institute of Science and Technology, Taipei, Taiwan (Lir)

Address correspondence to C. H. Wang at the address above, or e-mail: chingho{at}ntu.edu.tw.

Molecular sexing of the diversified avian family Strigidae is difficult. Sex identification using the intron length difference between W and Z chromosomal CHD1 genes, as visualized by agarose gel electrophoreses, often produces ambiguous results. Here we describe a simple method for sexing a variety of Strigidae species using oligonucleotide microarrays, on which several sex-specific probes operated complementarily or in concert. The sex of 8 owl species was identified clearly on the microarrays through sequence recognition. This sequence-directed method can be easily applied to a wider range of Strigidae species.

Corresponding Editor: Oliver Ryder

Received December 8, 2006
Accepted October 12, 2007


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