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Journal of Heredity Advance Access published online on March 15, 2008
Journal of Heredity, doi:10.1093/jhered/esn007
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© The American Genetic Association. 2008. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org.

Protein Polymorphism between 2 Picea abies Populations Revealed by 2-Dimensional Gel Electrophoresis and Tandem Mass Spectrometry

Cristina-Maria Valcu, Céline Lalanne, Gerhard Müller-Starck, Christophe Plomion, and Katja Schlink

From the Section of Forest Genetics, Technische Universität München, Am Hochanger 13, D-85354 Freising-Weihenstephan, Germany (Valcu, Müller-Starck, and Schlink); and the UMR Biogeco 1202, INRA, Equipe de Génétique, Cestas, Cedex, France (Lalanne and Plomion)

Address correspondence to C.-M. Valcu at the address above, or e-mail: valcu{at}wzw.tum.de.

In species with high gene flow and consequent low interpopulation differentiation over wide geographic ranges, differential gene expression along ecological gradients often reveals adaptive significance. We investigated potential differences in protein expression between Picea abies ecotypes adapted to contrasting altitude conditions. Protein expression patterns were compared between needles and roots of 2-month-old P. abies seedlings by means of 2-dimensional electrophoresis. Proteins exhibiting differential expression between the 2 ecotypes were analyzed by tandem mass spectrometry. A total of 19 proteins exhibited qualitative or quantitative polymorphism between the 2 populations. These proteins exhibited organ-specific expression, and the level of interpopulation protein polymorphism was organ dependent. Among differentially expressed proteins, we identified proteins involved in photosynthesis, photorespiration, root tracheary element differentiation, and transmitochondrial membrane transport. Our results show that P. abies seedlings from locally adapted ecotypes exhibit consistent differences in protein expression. The expression polymorphism of some of these proteins has potential adaptive significance.

Corresponding Editor: David Wagner

Received May 20, 2007
Accepted December 18, 2007


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