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Journal of Heredity Advance Access published online on March 11, 2008
Journal of Heredity, doi:10.1093/jhered/esn012
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© The American Genetic Association. 2008. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org.

An Induced Mass Spawn of the Hermaphroditic Lion-Paw Scallop, Nodipecten subnodosus: Genetic Assignment of Maternal and Paternal Parentage

Jessica L. Petersen, Ana M. Ibarra, José L. Ramirez, and Bernie May

From the Genomic Variation Laboratory, Department of Animal Science, University of California, One Shields Avenue, Davis, CA 95616 (Petersen and May); and the Aquaculture Genetics Laboratory, Centro de Investigaciones Biologicas del Noroeste S.C., A.P. 128, La Paz B.C.S., Mexico 23000 (Ibarra and Ramirez)

Address correspondence to J. L. Petersen at the address above, or e-mail: jlpetersen{at}ucdavis.edu.

The Pacific lion-paw scallop is commonly propagated for aquaculture by induced mass spawns of few individuals. Parentage of a mass spawn of this species has not been evaluated nor has the maternal and paternal contribution of each of these functional hermaphrodites to the progeny. Genotypes of 6 spawners and 374 resulting progeny at 6 microsatellite loci were coupled with mitochondrial DNA sequencing to assign maternal and paternal parentage. After the identification of a high proportion of null alleles (9.7%), microsatellite data revealed that 51.7% of the progenies were full siblings, with a significant, unequal contribution of the 6 spawners to the progeny. Three progenies were the result of self-fertilization. All spawners contributed paternally (though unequally); however, 2 spawners were the maternal parents of all but 7 progenies resulting in a variance effective population size of 3.52. DNA sequencing confirmed 4 microsatellite mutations within 4476 alleles scored, all in the paternal germ line. With minor exception, the loci conformed to Mendelian rules of segregation when null alleles were accounted for, and 2 loci were found to be linked. These results lend insight to the genetic composition of induced mass spawns and provide a basis for the development of more effective spawning techniques.

Corresponding Editor: Scott Baker

Received August 14, 2007
Accepted January 11, 2008


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