Skip Navigation



Journal of Heredity Advance Access published online on February 28, 2008
Journal of Heredity, doi:10.1093/jhered/esn013
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Buckner, B.
Right arrow Articles by Janick-Buckner, D.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Buckner, B.
Right arrow Articles by Janick-Buckner, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The American Genetic Association. 2008. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org.

Expression and Nucleotide Diversity of the Maize RIK Gene

Brent Buckner, Kayleigh A. Swaggart, Cheryl C. Wong, Heath A. Smith, Kelsey M. Aurand, Michael J. Scanlon, Patrick S. Schnable, and Diane Janick-Buckner

From the Department of Biology, Truman State University, 100 East Normal Street, Kirksville, MO 63501 (Buckner, Swaggart, Wong, Smith, Aurand, and Janick-Buckner); the Department of Plant Biology, Cornell University, Ithaca, NY 14850 (Scanlon); the Department of Agronomy, Iowa State University, Ames, IA 50011 (Schnable); and the Department of Genetics, Developmental Biology and Cell Biology, Iowa State University, Ames, IA 50011 (Schnable)

Address correspondence to Dr B. Buckner at the address above, or e-mail: bbuckner{at}truman.edu.

The K homology (KH) domain is a conserved sequence present in a wide variety of RNA-binding proteins. The rough sheath2–interacting KH domain (RIK) protein of maize has been implicated in the maintenance of the repressed chromatin state of knox genes during leaf primordia initiation. The amino acid sequences of the publicly available plant RIK proteins contain a splicing factor 1 (SF1)–like KH domain core sequence motif that distinguishes them from all other SF1-like KH domain–containing proteins. We demonstrate that the maize RIK gene exhibits surprisingly little nucleotide sequence diversity among Zea species and subspecies. Microarray hybridization experiments demonstrate that RIK has a higher level of expression in the shoot apical meristem as compared with 14-day seedling. Reverse transcriptase–polymerase chain reaction analysis of RIK indicates that the gene is expressed in many tissues, albeit at lower levels in older leaf samples. Taken together, these data suggest that the RIK protein may be involved in the maintenance of an inactive chromatin state of knox and possibly other genes in nonmeristematic tissues.

Corresponding Editor: Susan Gabay-Laughnan

Received September 28, 2007
Accepted January 11, 2008


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.