Skip Navigation



Journal of Heredity Advance Access published online on February 28, 2008
Journal of Heredity, doi:10.1093/jhered/esn014
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Material
Right arrow All Versions of this Article:
99/4/396    most recent
esn014v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Törjék, O.
Right arrow Articles by Altmann, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Törjék, O.
Right arrow Articles by Altmann, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The American Genetic Association. 2008. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org.

Construction and Analysis of 2 Reciprocal Arabidopsis Introgression Line Populations

Ottó Törjék*, Rhonda C. Meyer*, Maik Zehnsdorf, Melanie Teltow, Georg Strompen, Hanna Witucka-Wall, Anna Blacha, and Thomas Altmann

From the Department of Genetics, Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Strasse 24-25, 14476 Potsdam-Golm, Germany (Törjék, Teltow, Strompen, Witucka-Wall, and Altmann); and the Max-Planck-Institute of Molecular Plant Physiology, Am Muehlenberg 1, 14476 Golm, Germany (Meyer, Zehnsdorf, Blacha, and Altmann). Ottó Törjék is now at Biological Resource Center, Institute of Plant Biology, Cell Division Cycle and Stress Adaptation Group, Temesvári krt. 62, PO Box 521, H-6701 Szeged, Hungary

Address correspondence to R. C. Meyer at the address above, or e-mail: meyer{at}mpimp-golm.mpg.de.

Two new large reciprocal sets of introgression lines (ILs) were created between the Arabidopsis accessions Col-0 and C24. In both sets (78 ILs with Col-0 background and 62 ILs with C24 background), the donor segments cover almost the entire genome with an average substitution size of 18.3 cM. In addition to the basic sets of ILs, further subILs were developed for 2 genomic regions allowing better mapping resolution. SubILs carrying donor segments with candidate genes for flowering time and reduced fertility were used to demonstrate the usefulness of the reciprocal ILs for quantitative trait loci detection and fine mapping. For subIL development at high resolution around the reduced fertility locus, we used modified CelI-based assays in one-well format for both marker development and genotyping. This serves as a very flexible and cost-effective approach.

* These authors contributed equally to the work.

Corresponding Editor: James Hamrick

Received July 11, 2007
Accepted January 11, 2008


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.