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Journal of Heredity Advance Access published online on February 28, 2008
Journal of Heredity, doi:10.1093/jhered/esn014
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© The American Genetic Association. 2008. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org.

Construction and Analysis of 2 Reciprocal Arabidopsis Introgression Line Populations

Ottó Törjék*, Rhonda C. Meyer*, Maik Zehnsdorf, Melanie Teltow, Georg Strompen, Hanna Witucka-Wall, Anna Blacha, and Thomas Altmann

From the Department of Genetics, Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Strasse 24-25, 14476 Potsdam-Golm, Germany (Törjék, Teltow, Strompen, Witucka-Wall, and Altmann); and the Max-Planck-Institute of Molecular Plant Physiology, Am Muehlenberg 1, 14476 Golm, Germany (Meyer, Zehnsdorf, Blacha, and Altmann). Ottó Törjék is now at Biological Resource Center, Institute of Plant Biology, Cell Division Cycle and Stress Adaptation Group, Temesvári krt. 62, PO Box 521, H-6701 Szeged, Hungary

Address correspondence to R. C. Meyer at the address above, or e-mail: meyer{at}mpimp-golm.mpg.de.

Two new large reciprocal sets of introgression lines (ILs) were created between the Arabidopsis accessions Col-0 and C24. In both sets (78 ILs with Col-0 background and 62 ILs with C24 background), the donor segments cover almost the entire genome with an average substitution size of 18.3 cM. In addition to the basic sets of ILs, further subILs were developed for 2 genomic regions allowing better mapping resolution. SubILs carrying donor segments with candidate genes for flowering time and reduced fertility were used to demonstrate the usefulness of the reciprocal ILs for quantitative trait loci detection and fine mapping. For subIL development at high resolution around the reduced fertility locus, we used modified CelI-based assays in one-well format for both marker development and genotyping. This serves as a very flexible and cost-effective approach.

* These authors contributed equally to the work.

Corresponding Editor: James Hamrick

Received July 11, 2007
Accepted January 11, 2008


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