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Journal of Heredity Advance Access published online on September 26, 2009
Journal of Heredity, doi:10.1093/jhered/esp080
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© The American Genetic Association. 2009. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org.

Brief Communication

Microsatellite Marker Development and Mendelian Analysis in the Matschie's Tree Kangaroo (Dendrolagus matschiei)

Thomas J. McGreevy, Jr, Lisa Dabek, and Thomas P. Husband

From the Department of Natural Resources Science, Coastal Institute, University of Rhode Island, Kingston, RI 02881 (McGreevy Jr, Dabek, and Husband); and the Tree Kangaroo Conservation Program, Department of Conservation, Woodland Park Zoo, Seattle, WA (Dabek)

Address correspondence to Thomas J. McGreevy Jr at the address above, or e-mail: tjmcg{at}mail.uri.edu.

Matschie's tree kangaroo (Dendrolagus matschiei) is an endangered arboreal macropodid endemic to the Huon Peninsula, Papua New Guinea (PNG). We developed 5 microsatellite markers for D. matschiei, which are the first markers developed for Dendrolagus. We screened 17 additional markers that were developed for other marsupial taxa and identified 3 that were polymorphic in D. matschiei. We estimated allelic and genetic diversity with the set of 8 markers by analyzing 22 D. matschiei from Wasaunon on the Huon Peninsula, PNG. The number of alleles ranged from 2 to 9 and expected heterozygosity ranged from 0.440 to 0.794. We tested for null alleles and Mendelian inheritance by analyzing 19 pairs of D. matschiei parents and offspring from Association of Zoos and Aquariums institutions. Null alleles were not detected and Mendelian inheritance was followed for all 8 markers. We also evaluated the reliability of using the markers to amplify DNA extracted from D. matschiei fecal samples and the ability of the markers to amplify DNA samples from Goodfellow's tree kangaroo (Dendrolagus goodfellowi ssp.), Doria's tree kangaroo (Dendrolagus dorianus ssp.), and Grizzled tree kangaroo (Dendrolagus inustus ssp.). Microsatellite markers can be used to inform management decisions to conserve D. matschiei in captivity and the wild.

Key Words: association of zoos and aquariums • conservation genetics • endangered species • fecal DNA • noninvasive genotyping • Papua New Guinea

Corresponding Editor: Robert C. Fleischer

Received April 25, 2009
Revised August 3, 2009
Accepted August 17, 2009


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