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The Journal of Heredity 1984:75(6):431-434
© 1984 The American Genetic Association 75:431-434

research-article

Replication banding patterns in the chromosomes of the rainbow trout

Mary E. Delany, and Stephen E. Bloom

Department of Poultry and Avian Sciences, Cornell University 214 Rice Hall, Ithaca NY, 14853

Abstract

Replication bands were induced in the metaphase chromosomes of the rainbow trout (Salmo gairdneri, 2n = 60, NF = 104). The fluorescent banding patterns were generated by incorporation of 5-bromodeoxyuridine (BrdU) into the DNA during S phase of the cell cycle. This was accomplished by in vivo administration of BrdU every 5 hours for 25 hours. Chromosomes were harvested during the following metaphase and stained by the fluorochrome Hoechst 33258. The reproducible induction of replication banding patterns suggested that rainbow trout DNA is replicated via temporal organization of repilcon clusters. Thirty discrete chromosome pairs were identified in the karyotype based on fluorescent banding pattern, chromosome size, and centromere position. Observations from this study suggested that the majority of the metacentrics were formed by Robertsonian translocations of nonhomologous rather than homologous (homeologous) arms during the evolution of the rainbow genome. Several sets of chromosome arms exhibited similar banding patterns. These similarities may reflect homeology between tetrasomic arms. The Identification of chromosome arms by their replication pattern permits further evaluation of the chromosome structure changes that have occurred during the evolution of this species as well as the other members of the tetraploid derivative Salmonidae. Modifications of this technique should prove useful for chromosome Identification in other fish taxa


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