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The Journal of Heredity 1995:86(5):354-363
© 1995 The American Genetic Association 86:354-363

research-article

Genetic Variation at Minisatellite DNA Loci Among North Pacific Populations of Steelhead and Rainbow Trout (Oncorhynchus mykiss)

E. B. Taylor

Canadian Department of Fisheries and Oceans, Biological Sciences Branch, Pacific Biological Station Nanaimo, British Columbia, Canada

Abstract

Genetic variation at Minisatellite DNA, or variable number tandem repeat (VNTR), loci is widely studied in the context of animal breeding and pedigree analyses, but comparatively little information exists on the levels of variation at such loci in natural populations. I examined allelic variability at two VNTR loci (Ssa1 and T34) by Southern hybridization analyses within and between populations of steelhead and rainbow trout, the sea-run and freshwater resident life-history forms of Oncorhynchus mykiss, from eight populations tributary to the northeast Pacific Ocean. Single-locus expected heterozygosities ranged from an average of 61% (Ssa1) to 80% (T34) in the eight populations, and no significant departures from Hardy-Weinberg expected genotype frequencies were detected. Eighteen putative allelic fragments were resolved in the 267 steelhead and rainbow trout examined at Ssa1(molecular weight range, 3.6–9.5 kilobase pairs (kbp)], and 26 alleles were resolved at T34(1.7–9.4kbp). At Ssa1, however, one allele accounted for 58% of all alleles scored and at T34 three alleles accounted for 72% of those scored. Allele frequencies at both loci were stable within two populations sampled over successive years, but varied significantly between populations within watersheds, and large frequency differences were detected between major geographic areas (e.g., Alaska versus British Columbia). Neighbor-joining analyses of genetic distances among populations accompanied by bootstrap analysis provided strong support(>70%) for clustering of populations by geographic region, as well as for a major genetic distinction (100% bootstrap support) between interior Frasher River populations of rainbow trout and coastal steelhead trout.


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