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The Journal of Heredity 2001:92(4)
© 2001 The American Genetic Association 92:357-359

Brief Communication

Non-Sex-Linked, Nuclear Cleaved Amplified Polymorphic Sequences in Silene latifolia

V. Laporte, and D. Charlesworth

From the Institute of Cell, Animal, and Population Biology (ICAPB), University of Edinburgh, Ashworth Laboratory, King's Buildings, West Mains Road, Edinburgh EH9 3JT, UK.

Address correspondence to V. Laporte and D. Charlesworth at the address above.

Cleaved amplified polymorphic sequences (CAPS) were identified for five nuclear genes in Silene latifolia. By using published cDNA sequences of S. latifolia, pairs of primers were designed to amplify small regions of six nuclear genes. Targeted regions were successfully amplified, two of which included introns. By using direct sequencing of diploid individuals, suitable polymorphic sites for CAPS markers were rapidly detected in five of six of these gene regions, thus avoiding the tedious screening of a large panel of restriction enzymes. Using controlled progenies, we have also shown that all these CAPS markers segregated independently of the sex phenotype, thus demonstrating that the genes analyzed here are not located in the nonrecombining region of the sex chromosomes.


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Home page
 Mol Biol EvolHome page
D. A. Filatov
Substitution Rates in a New Silene latifolia Sex-Linked Gene, SlssX/Y
Mol. Biol. Evol., March 1, 2005; 22(3): 402 - 408.
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