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Journal of Heredity Advance Access originally published online on April 9, 2007
Journal of Heredity 2007 98(3):202-210; doi:10.1093/jhered/esm015
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© The American Genetic Association. 2007. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org.

Microsatellite Isolation and Linkage Group Identification in the Yellow Fever Mosquito Aedes aegypti

Eric W. Chambers, Jennifer K. Meece, James A. McGowan, Diane D. Lovin, Ryan R. Hemme, Dave D. Chadee, Kevin McAbee, Susan E. Brown, Dennis L. Knudson, and David W. Severson

From the Center for Global Health and Infectious Diseases, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556 (Chambers, Meece, McGowan, Lovin, Hemme, McAbee, and Severson); the Department of Life Sciences, University of the West Indies, St Augustine, Trinidad, West Indies (Chadee); and the Department of Bioagricultural Sciences and Pest Management, College of Agricultural Sciences, Colorado State University, Fort Collins, CO 80523 (Brown and Knudson)

Address correspondence to: Dr. D. W. Severson at the address above, or e-mail: severson.1{at}nd.edu.

Microsatellites have proved to be very useful as genetic markers, as they seem to be ubiquitous and randomly distributed throughout most eukaryote genomes. However, our laboratories and others have determined that this paradigm does not necessarily apply to the yellow fever mosquito Aedes aegypti. We report the isolation and identification of microsatellite sequences from multiple genomic libraries for A. aegypti. We identified 6 single-copy simple microsatellites from 3 plasmid libraries enriched for (GA)n, (AAT)n, and (TAGA)n motifs from A. aegypti. In addition, we identified 5 single-copy microsatellites from an A. aegypti cosmid library. Genetic map positions were determined for 8 microsatellite loci. These markers greatly increase the number of microsatellite markers available for A. aegypti and provide additional tools for studying genetic variability of mosquito populations. Additionally, most A. aegypti microsatellites are closely associated with repetitive elements that likely accounts for the limited success in developing an extensive panel of microsatellite marker loci.

Corresponding Editor: Ross MacIntyre

Received November 28, 2005
Accepted January 31, 2007


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