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Journal of Heredity Advance Access published online on December 14, 2004
Journal of Heredity, doi:10.1093/jhered/esi015
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© 2005 The American Genetic Association
Received November 25, 2003
Accepted July 23, 2004

Computer Note

Expeditor: A Pipeline for Designing Primers Using Human Gene Structure and Livestock Animal EST Information

Z.-L. Hu 1*, K. Glenn 1, A. M. Ramos 1, C. J. Otieno 1, J. M. Reecy 1, and M. F. Rothschild 1

1 From the Department of Animal Science, 2255 Kildee Hall, Center for Integrated Animal Genomics, Iowa State University, Ames, IA 50011

* To whom correspondence should be addressed.
Z.-L. Hu, E-mail: zhu{at}iastate.edu


  Abstract

We have developed software, called Expeditor, that can be used to combine known gene structure information from human and coding sequence information from farm animal species for a streamlined primer design in target farm animal species. This software has many utilities, which include PCR-based SNP discovery for identification of genes/markers associated with economically important traits in farm animals, comparative mapping analysis, and evolution studies. The use of this software helps minimize tedious manual operations and reduces the chance of errors by more conventional approaches.


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