Skip Navigation



Journal of Heredity Advance Access published online on July 1, 2005
Journal of Heredity, doi:10.1093/jhered/esi076
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
96/5/576    most recent
esi076v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Salgado, C.
Right arrow Articles by García-Dorado, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Salgado, C.
Right arrow Articles by García-Dorado, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The American Genetic Association. 2005. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org.
Received January 15, 2005
Accepted March 17, 2005

Brief Communication

Inferences on the Role of Insertion in a Mutation Accumulation Experiment with Drosophila melanogaster Using RAPDs

C. Salgado 1, B. Nieto 1, M. A. Toro 2, C. López-Fanjul 3, and A. García-Dorado 3*

1 From the Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid
2 From the Departamento de Mejora Genética Animal, INIA, 28040 Madrid
3 From the Departamento de Genética, Facultad de Biología, Universidad Complutense, 28040 Madrid

* To whom correspondence should be addressed.
A. García-Dorado, E-mail: augardo{at}bio.ucm.es


  Abstract

The genetic variability for RAPDs band pattern was studied in a set of 157 mutation accumulation (MA) lines of Drosophila melanogaster. These MA lines were derived from the same isogenic base population and subsequently maintained by full-sib mating during 132 generations. The ancestral pattern of the original isogenic base can be unambiguously established as the consensus pattern of the MA lines and, because these lines are expected to be homozygous, dominance for band pattern is not a concern. Only repeatable changes in band pattern were considered. The number of ancestral bands detected implies that nine-nucleotide targets are enough for repeatable PCR amplification. Compared with the ancestral pattern, one MA line lost one band and two MA lines gained a new one. These results can be accounted for by the insertion of transposable elements occurring at a rate 0.07 < i < 0.21 per whole haploid genome and generation. This range is typical for Drosophila and consistent with the previously observed mobility for the roo family, supporting the generality of previous estimates of spontaneous mutation rates for morphological and fitness traits based on these MA lines. The sequence of one of the new bands suggests that the Idefix family is also active in the lines.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 GeneticsHome page
M. Papaceit, V. Avila, M. Aguade, and A. Garcia-Dorado
The Dynamics of the roo Transposable Element In Mutation-Accumulation Lines and Segregating Populations of Drosophila melanogaster
Genetics, September 1, 2007; 177(1): 511 - 522.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.