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Journal of Heredity Advance Access published online on June 30, 2005
Journal of Heredity, doi:10.1093/jhered/esi085
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© The American Genetic Association. 2005. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org.

Article

Establishment of a Cell Line Derived from a Canine Prostate Carcinoma with a Highly Rearranged Karyotype

S. Winkler 1, H. Murua Escobar 2, N. Eberle 2, N. Reimann-Berg 1, I. Nolte 2, and J. Bullerdiek 1*

1 From the Centre for Human Genetics, University of Bremen, Leobener Strasse ZHG, 28359 Bremen, Germany
2 From the Small Animal Clinic, School of Veterinary Medicine, Bischofsholer Damm 15, 30173 Hanover, Germany

* To whom correspondence should be addressed.
J. Bullerdiek, E-mail: bullerd{at}uni-bremen.de


  Abstract

Akin to the situation in humans, dogs are frequently affected by tumors of the prostate. The malignancies share many similarities between both species, for example, median age at the onset of the disease and metastatic behavior. In human prostatic tumor samples, investigations of prepared metaphase spreads showed a variety of chromosomal aberrations, with trisomies of chromosomes 7, 8, and 17 as the leading cytogenetic abnormalities. In this article we present one case of a canine adenocarcinoma of the prostate, including clinical examination and establishment of a cell line from a tumor sample obtained from the affected 10-year-old male Briard. Searching for similarities between both species in respect to chromosomal changes within the tumor samples, we investigated prepared metaphases of the canine cell line cytogenetically. These investigations presented a highly rearranged karyotype showing a large biarmed marker consisting of material from chromosomes 1 and 2 in addition to centromeric fusions between dog chromosomes 1 and 5 that both could be identified in every metaphase investigated, while centric fusions of chromosomes 4 and 5 occurred in up to 50% of the metaphases. The cell line grew very well and showed evidence of being spontaneously immortalized when it crossed the 20th passage.


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