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Journal of Heredity Advance Access published online on July 1, 2005
Journal of Heredity, doi:10.1093/jhered/esi089
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© The American Genetic Association. 2005. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org.
Received January 12, 2004
Accepted March 25, 2005

Brief Communication

Identification of Microsatellite DNA Markers for the Giant Anteater Myrmecophaga tridactyla

J. E. Garcia 1*, L. A. Vilas Boas 2, M. V. F. Lemos 3, E. G. de Macedo Lemos 4, and E. P. B. Contel 5

1 From the Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirão Preto, SP, Brazil; Current address: Departamento de Bioquímica e Biotecnologia, Universidade Estadual de Londrina, P.O. Box 6001, 86051-990, Londrina, PR, Brazil
2 Departamento de Biologia Geral, Universidade Estadual de Londrina, P.O. Box 6001, 86051-990, Londrina, PR, Brazil; Departamento de Biologia Geral, Instituto de Biologia, Universidade Federal da Bahia. Rua Barão de Geremoabo, 40170-290, Salvador, BA, Brazil
3 Departamento de Biologia Aplicada à Agropecuária, Faculdade de Ciências Agrárias e Veterinárias, Campus de Jaboticabal, Universidade Estadual Paulista, Via de Acesso Prof. Paulo Donato Castellane, km 5, 14870-900, Jaboticabal, SP, Brazil
4 Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, Campus de Jaboticabal, Universidade Estadual Paulista, Via de Acesso Prof. Paulo Donato Castellane, km 5, 14870-900, Jaboticabal, SP, Brazil
5 From the Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirão Preto, SP, Brazil

* To whom correspondence should be addressed.
J. E. Garcia, E-mail: jegarcia{at}uel.br


  Abstract

The giant anteater (Myrmecophaga tridactyla) is found from Belize and Guatemala to Paraguay and Argentina. Its conservation status is considered vulnerable by IUCN. Here we report the isolation and characterization of six microsatellite loci. Positive loci for (GT)n were isolated using a magnetic bead hybridization selection protocol. The number of alleles per locus as well as the heterozygosity and PCR conditions are described. These loci will be useful for studying population structure, genetic diversity, and paternity in M. tridactyla wild populations.


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