Skip Navigation



Journal of Heredity Advance Access published online on August 31, 2005
Journal of Heredity, doi:10.1093/jhered/esi095
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
96/5/603    most recent
esi095v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Freeman, J. L.
Right arrow Articles by Rayburn, A. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Freeman, J. L.
Right arrow Articles by Rayburn, A. L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The American Genetic Association. 2005. All rights reserved. For permissions, please email: journals.permissions@oupjournals.org.
Received December 10, 2004
Accepted May 26, 2005

Brief Communication

Localization of Repetitive DNA Sequences on in vitro Xenopus laevis Chromosomes by Primed in situ Labeling (PRINS)

J. L. Freeman 1 and A. L. Rayburn 1*

1 From the University of Illinois, Department of Crop Sciences, 1201 W. Gregory Dr., 320 ERML, Urbana, IL 61801

* To whom correspondence should be addressed.
A. L. Rayburn, E-mail: arayburn{at}uiuc.edu


  Abstract

Xenopus laevis is an important reference model organism used in many vertebrate studies. Gene mapping in X. laevis, in comparison to other reference organisms, is in its early stages. Few studies have been conducted to localize DNA sequences on X. laevis chromosomes. Primed in situ labeling (PRINS) is a recently developed innovative tool that has been used to locate specific DNA sequences in various organisms. PRINS has been reported to have increased sensitivity compared to other in situ hybridization techniques. In the present study, PRINS was first used to label the location of telomeres at the ends of in vitro X. laevis chromosomes. The terminal location was as expected from in vivo reports, however, the overall amount seemed to decrease in the in vitro chromosomes. Once the PRINS technique was optimized, this technique was used to determine the chromosomal location of the satellite 1 repetitive sequence, which is an important sequence in X. laevis development. The sequence was observed on the interstitial regions of the majority of the chromosomes similar to the in vivo locations reported. In contrast to the telomeric sequence, the amount of sequence appeared to increase in the satellite 1 sequence. PRINS was found to be useful in the localization of repetitive DNA sequences in the X. laevis genome.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.