Skip Navigation



Journal of Heredity Advance Access published online on September 8, 2005
Journal of Heredity, doi:10.1093/jhered/esi105
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
96/7/821    most recent
esi105v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Burton-Wurster, N.
Right arrow Articles by Lust, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Burton-Wurster, N.
Right arrow Articles by Lust, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The American Genetic Association. 2005. All rights reserved. For permissions, please email: journals.permissions@oupjournals.org.

Article

Genes in Canine Articular Cartilage That Respond to Mechanical Injury: Gene Expression Studies With Affymetrix Canine GeneChip

N. Burton-Wurster 1*, R. G. Mateescu 1, R. J. Todhunter 2, K. M. Clements 3, Q. Sun 4, V. Scarpino 1, and G. Lust 1

1 From the Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853
2 Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853
3 From the Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853; K. M. Clements is currently at AstraZeneca, Alderley Park, Macclesfield, Cheshire, UK SK10 4TG
4 Theory Center, Cornell University, Ithaca, NY 14853

* To whom correspondence should be addressed.
N. Burton-Wurster, E-mail: niw1{at}cornell.edu


  Abstract

The Affymetrix canine GeneChip with 23,836 probe sets was used to look for cartilage genes that are significantly altered in response to mechanical impact. The model using canine articular cartilage explants loaded in vitro has been described previously (Chen et al., J Orthop Res 19:703-711, 2001). It is our hypothesis that genes that are activated or repressed in articular cartilage after impact injury initiate cartilage degeneration, leading to osteoarthritis in dogs. Gene expression of known cartilage genes was generally consistent with cartilage biology. A total of 528 genes were significantly (P < .01) up- or down- regulated in response to mechanical damage. After applying the strict Bonferroni correction, 172 remained significantly affected. One of these genes, MIG-6/gene 33, was chosen for verification by real- time quantitative reverse transcriptase polymerase chain reaction (RT-PCR). A 3.8- fold increase in expression was confirmed, consistent with the microarray chip data. Deficiencies in the current annotation of the canine chip are discussed. Gene expression studies with the Affymetrix canine GeneChip are potentially valuable, but await more complete annotation.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.