Skip Navigation



Journal of Heredity Advance Access published online on January 4, 2006
Journal of Heredity, doi:10.1093/jhered/esj007
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
97/1/39    most recent
esj007v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kato, A.
Right arrow Articles by Birchler, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kato, A.
Right arrow Articles by Birchler, J. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The American Genetic Association. 2006. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org.
Received July 12, 2005
Accepted October 11, 2005

Article

Induction of Tetraploid Derivatives of Maize Inbred Lines by Nitrous Oxide Gas Treatment

Akio Kato 1 * and James A. Birchler 2

1 From 117 Tucker Hall, University of Missouri, Columbia, MO 65211; The Faculty of Agriculture, Kyoto Prefectural University, Kyoto-shi, Sakyo-ku, Shimogamo Hangi-cho 1-5, Kyoto 606-0823, Japan
2 From 117 Tucker Hall, University of Missouri, Columbia, MO 65211

* To whom correspondence should be addressed.
Akio Kato, E-mail: katoa{at}kpu.ac.jp


  Abstract

Maize (Zea mays L.) is a model organism for various genetic and physiological studies. Induction of autotetraploid lines from elite inbred lines is valuable for investigating gene dosage effects on the molecular level. We applied nitrous oxide gas at the time of fertilization (30-36 h after pollination) for 20 h on maize inbred line Oh43. The nitrous oxide gas treatment between pressures of 600-1000 kPa proved to be effective in inducing tetraploids. The treatment also significantly increased the rates of germless and shriveled kernels. Twelve inbred lines were treated with nitrous oxide gas for 20 h at either 800 or 900 kPa pressures, 30 or 36 h after pollination. Although tetraploid or tetraploid class aneuploid plants from 9 of 12 inbreds tested were successfully generated, only six genotypes produced progenies. The successful tetraploid inbred lines were from the A188, B73, H99, Oh43, Stock 6, and W22 genetic backgrounds. Aneuploids, plants with broken chromosomes and chimeras, were also found among the treated materials.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.