Journal of Heredity Advance Access published online on July 13, 2007
Journal of Heredity, doi:10.1093/jhered/esm036
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Agouti Sequence Polymorphisms in Coyotes, Wolves and Dogs Suggest Hybridization
From the Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, Canada S7N 5A8 (Schmutz, Berryere, and Reddick); the Department of Anthropology, McMaster University, Hamilton, Ontario, Canada L8S 4L9 (Barta); and the Centre for Studies in Agriculture, Law and the Environment, University of Saskatchewan, Saskatoon, Canada S7N 5A8 (Schmutz)
Address correspondence to S. M. Schmutz at the address above, or e-mail: sheila.schmutz{at}usask.ca.
Domestic dogs have been shown to have multiple alleles of the Agouti Signal Peptide (ASIP) in exon 4 and we wished to determine the level of polymorphism in the common wild canids of Canada, wolves and coyotes, in comparison. All Canadian coyotes and most wolves have banded hairs. The ASIP coding sequence of the wolf did not vary from the domestic dog but one variant was detected in exon 4 of coyotes that did not alter the arginine at this position. Two other differences were found in the sequence flanking exon 4 of coyotes compared with the 45 dogs and 1 wolf. The coyotes also demonstrated a relatively common polymorphism in the 3' UTR sequence that could be used for population studies. One of the ASIP alleles (R96C) in domestic dogs causes a solid black coat color in homozygotes. Although some wolves are melanistic, this phenotype does not appear to be caused by this same mutation. However, one wolf, potentially a dog–wolf hybrid or descendant thereof, was heterozygous for this allele. Likewise 2 coyotes, potentially dog–coyote or wolf–coyote hybrid descendants, were heterozygous for the several polymorphisms in and flanking exon 4. We could conclude that these were coyote–dog hybrids because both were heterozygous for 2 mutations causing fawn coat color in dogs.
Corresponding Editor: Stephen J. O'Brien
Received April 10, 2006
Accepted March 9, 2007