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Journal of Heredity Advance Access published online on July 23, 2007
Journal of Heredity, doi:10.1093/jhered/esm055
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© The American Genetic Association. 2007. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org.

Genome Annotation Resource Fields—GARFIELD: A Genome Browser for Felis catus

Joan U. Pontius, and Stephen J. O'Brien

From the Laboratory of Genomic Diversity, Basic Research Program, SAIC-Frederick, Inc., NCI-Frederick, Frederick, MD 21702 (Pontius); and the Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD 21702 (O'Brien)

Address correspondence to J. U. Pontius at the address above, or e-mail: pontiusj{at}ncifcrf.gov.

Annotation features from the 1.9-fold whole-genome shotgun (WGS) sequences of domestic cat have been organized into an interactive web application, Genome Annotation Resource Fields (GARFIELD) (http://lgd.abcc.ncifcrf.gov) at the Laboratory of Genomic Diversity and Advanced Biomedical Computing Center (ABCC) at The National Cancer Institute (NCI). The GARFIELD browser allows the user to view annotations on a per chromosome basis with unplaced contigs provided on placeholder chromosomes. Various tracks on the browser allow display of annotations. A Genes track on the browser includes 20 285 regions that align to genes annotated in other mammalian genomes: Homo sapiens, Pan troglodytes, Mus musculus, Rattus norvegicus, Bos taurus, and Canis familiaris. Also available are tracks that display the contigs that make up the chromosomes and representations of their GC content and repetitive elements as detected using the RepeatMasker (http://www.repeatmasker.org). Data from the browser can be downloaded in FASTA and GFF format, and users can upload their own data to the display. The Felis catus sequences and their chromosome assignments and additional annotations incorporate data analyzed and produced by a multicenter collaboration between NCI, ABCC, Agencourt Biosciences Corporation, Broad Institute of Harvard and Massachusetts Institute of Technology, National Human Genome Research Institute, National Center for Biotechnology and Information, and Texas A&M.

This paper was delivered at the 3rd International Conference on the Advances in Canine and Feline Genomics, School of Veterinary Medicine, University of California, Davis, CA, August 3–5, 2006.

Corresponding Editor: Urs Giger


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