Journal of Heredity Advance Access first published online on July 23, 2007
This version published online on July 31, 2007
Journal of Heredity, doi:10.1093/jhered/esm063
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Development of Y Chromosome Intraspecific Polymorphic Markers in the Felidae
From the Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD 21702-1201 (Luo, Johnson, David, Menotti-Raymond, Beck, Yuhki, Pecon-Slattery, and O'Brien); the Department of Fisheries, Wildlife and Conservation Biology, University of Minnesota, St Paul, MN 55108 (Luo and Smith); Department of Animal Biology and Genetics, University of Florence, Firenze, Italy (Stanyon); and the Key Laboratory for Biodiversity Science and Ecological Engineering, Ministry of Education, Institute of Ecology, Beijing Normal University, Beijing 100875, People's Republic of China (Cai)
Address correspondence to S. J. O'Brien at the address above, or e-mail: obrien{at}ncifcrf.gov or to S.-J. Luo at the address above, or e-mail: luos{at}ncifcrf.gov.
Y chromosome haplotyping based on microsatellites and single nucleotide polymorphisms (SNPs) has proved to be a powerful tool for population genetic studies of humans. However, the promise of the approach is hampered in the majority of nonhuman mammals by the lack of Y-specific polymorphic markers. We were able to identify new male-specific polymorphisms in the domestic cat Felis catus and 6 additional Felidae species with a combination of molecular genetic and cytogenetic approaches including 1) identifying domestic cat male-specific microsatellites from markers generated from a male cat microsatellite-enriched genomic library, a flow-sorted Y cosmid library, or a Y-specific cat bacteria artificial chromosome (BAC) clone, (2) constructing microsatellite-enriched libraries from flow-sorted Y chromosomes isolated directly from focal wildcat species, and (3) screening Y chromosome conserved anchored tagged sequences primers in Felidae species. Forty-one male-specific microsatellites were identified, but only 6 were single-copy loci, consistent with the repetitive nature of the Y chromosome. Nucleotide diversity (
) of Y-linked intron sequences (2.1 kbp) was in the range of 0 (tiger) to 9.95 x 10–4 (marbled cat), and the number of SNPs ranged from none in the tiger to 7 in the Asian leopard cat. The Y haplotyping system described here, consisting of 4 introns (SMCY3, SMCY7, UTY11, and DBY7) and 1 polymorphic microsatellite (SMCY-STR), represents the first available markers for tracking intraspecific male lineage polymorphisms in Felidae species and promises to provide significant insights to evolutionary and population genetic studies of the species.
This paper was delivered at the 3rd International Conference on the Advances in Canine and Feline Genomics, School of Veterinary Medicine, University of California, Davis, CA, August 3–5, 2006.
Corresponding Editor: Urs Giger
The corresponding e-mail address for Dr. Luo has been corrected.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Navascues, O. J. Hardy, and C. Burgarella Characterization of Demographic Expansions From Pairwise Comparisons of Linked Microsatellite Haplotypes Genetics, March 1, 2009; 181(3): 1013 - 1019. [Abstract] [Full Text] [PDF]
|
|